Validation of a flow cytometry-based detection of γ-H2AX, to

‪Francesca Cappellini‬ - ‪Google Scholar‬

This result 4 Nov 2016 X, Gamma-H2AX; Isotype: Mouse IgG1, κ; Ave. Rating: Submit a X- Phosphorylated (Ser139) Antibody for Flow Cytometry 1. Prepare 70% Validation of a flow cytometry-based detection of γ-H2AX, to measure DNA damage for clinical applications. Artikel i vetenskaplig tidskrift, refereegranskad. A control for the day-to-day normalization of the flow cytometry γ-H2AX assay for clinical routine. Artikel i vetenskaplig tidskrift, refereegranskad. Författare.

pubmed.ncbi.nlm.nih.gov tissues. gamma-H2AX has already been investigated in a variety of cancer types, including breast, lung, colon, cervix, and ovary cancers. The prognostic value of gamma-H2AX is indicated in certain cancer types, such as breast or endometrial cancer, but further investigation is needed to establish gamma-H2AX as a prognostic marker. Phosphorylated H2AX (also termed, gamma-H2AX) functions to recruit and localize DNA repair proteins or cell cycle checkpoint factors to the DNA-damaged sites. In this way, phosphorylated H2AX promotes DNA repair and maintains genomic stability and thus helps prevent oncogenic transformations. To this end, we selected 14 well-known genotoxic compounds and compared them with 10 nongenotoxic chemicals, using CHO-9 cells because they are well characterized as to DNA repair and DDR. We quantified gamma-H2AX foci manually and automatically. In addition, total gamma-H2AX activation was determined by flow cytometry.

Simple Western: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Electropherogram image(s) of corresponding Simple Western lane view. Gamma H2AX antibody was used at 10 ug/ml dilution on Jurkat lysate(s).

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A control for the day-to-day normalization of the flow cytometry γ-H2AX assay for clinical routine. Artikel i vetenskaplig tidskrift, refereegranskad. Författare.

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Artikel i vetenskaplig tidskrift, refereegranskad. A control for the day-to-day normalization of the flow cytometry γ-H2AX assay for clinical routine. Artikel i vetenskaplig tidskrift, refereegranskad.

Bourton EC, Plowman PN, Zahir SA, Senguloglu GU, Serrai H, Bottley G, et al. Multispectral imaging flow cytometry reveals distinct frequencies of gamma-H2AX foci induction in DNA double strand break repair defective human cell lines. Cytometry A. 2012;81: 130–7. pmid:22170789 . View Article PubMed/NCBI Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle.
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The exposure to multiple CT scans causes more double strand breaks as compared to single scan. DNA damage can be studied by flow cytometric analysis of gamma-H2AX in human peripheral lymphocytes. assays for the sensitive measurement of g-H2AX, using both microscopy and ﬂow cytometry (21–25). Analysis of g-H2AX by microscopy is still considered to be the most sensitive detection method and may discriminate between differential g-H2AX responses with respect to drug type and cell population makeup (7). However, the In contrast, flow cytometry allows simple detection of gamma-H2AX in a large number of cells .

At present, flow cytometry is the most rapid method for detection of DSBs and cell viability. In this chapter, we provide our experience and methodological modification of flow cytometry protocol Measurement of c-H2AX by Flow Cytometry H2AX phosphorylation was analyzed by ﬂow cyto-metry analysis as previously described (23), with small modiﬁcations. After treatment, 1 mL of 0.1% BSA-PBS was added to the samples and PBMCs were pelleted (5 min at 2000g) followed by ﬁxation in 0.25% paraformaldehyde-PBS (8 3 106 cells/mL), for 10 min Antibody specific for gamma-H2AX is added and the positive cells are quantified for damage analysis, through flow cytometry. Although flow cytometry has been used to quantify DNA damage, it is still unexplored when it comes to low dose radiation and its effects,.
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70.00. 0.00%. Avg Ped RR (N=8). 94.88%. 99.29%.